IPTG诱导原理（原核表达）及实验步骤 | 私立大學五星教授網

為人工合成的乳糖操作組誘導物，其結構和乳醣類似，IPTG能在缺乏lacY基因下而有效地被運送，並可誘發β-半乳糖苷酶和接在此酵素後方之基因工程想表現的目標基因生成。

IPTG是一种分子生物学试剂，全称异丙基-β-D-硫代半乳糖苷（英語：Isopropyl β-D-1-thiogalactopyranoside），是異乳糖的结构类似物，能与乳糖抑制子结合，高效启动乳糖 ...

本文讲述了IPTG诱导原理/原核表达原理。在原核表达体系中，外源基因通常需要诱导剂IPTG诱导才能进行表达，本文详细讲述了常用诱导剂IPTG诱导表达的原理以及实验步骤。

IPTG or Isopropyl β-D-1-thiogalactopyranoside is a molecular analog of allolactose, which removes a repressor from the lac operon to induce gene expression.

Isopropyl β- d-1-thiogalactopyranoside (IPTG) is a molecular biology reagent. This compound is a molecular mimic of allolactose, a lactose metabolite that ...

Isopropyl β-d-1-thiogalactopyranoside (IPTG) is a molecular biology reagent. This compound is a molecular mimic of allolactose, a lactose metabolite that ...

IPTG即Isopropyl β-D-1-thiogalactopyranoside，也称Isopropyl β-D-thiogalactoside，中文名为异丙基-β-D-硫代半乳糖苷。分子式为C9H18O5S，分子量为238.30，CAS ...

由 L Gomes 著作 · 2020 · 被引用 52 次 — IPTG induction had a negative effect not only on growth but also on cellular viability of E. coli suspended cultures. Although IPTG was added in ...

由 A Marbach 著作 · 2012 · 被引用 203 次 — Here we report a systematic comparison of lac promoter induction by TMG and IPTG which focuses on the aspects inducer uptake, population heterogeneity and a ...

The most commonly used synthetic inductor of the Lac operon is IPTG since it is both active at very low concentrations and not subject to enzymatic degradation.