TA克隆 | 私立大學五星教授網

一、產品介紹： 應用：PCR 完作TA cloning，將PCR 片段接入載體，可同時做藍白篩選。

載體和目的基因進行上述配對之後，用DNA連接酶即可實現它們之間的共價連接。 Diagram of TA Cloning. TA克隆的圖示 ...

TA Cloning kits are available with a choice of Invitrogen pCR 2.1 and pCR II vectors. The T7 and Sp6 promoters of the pCR II vector allow in vitro transcription ...

TA cloning is a subcloning technique that avoids the use of restriction enzymes and is easier and quicker than traditional subcloning.

The TA cloning procedure begins by producing the insert in a PCR reaction using Taq polymerase, which adds a single A onto the ends of the PCR product (Fig.

T-vector cloning, or TA cloning, is a convenient method for cloning PCR products generated with Taq DNA Polymerase. The pGEM®-T vectors are a popular choice ...

TA Cloning Kits are designed for cloning Taq-amplified PCR products directly from a PCR reaction using an overnight ligation step. The kits use a pCR vector ...

由 MY Zhou 著作 · 2000 · 被引用 240 次 — Abstract. TA cloning is one of the simplest and most efficient methods for the cloning of PCR products. The procedure exploits the terminal transferase activity ...