Mammalian Protease Inhibitor Cocktail Preparation Protocol | 私立大學五星教授網

Table 6. Preparation of a general protease inhibitor cocktail ; Leupeptin, 10-100 μM, 2 mM, 18.9 mg/2 mL H2O (20 mM), 2 mL ; Pepstatin, 1 μM, 100 μM, 6.8 mg/10 mL ...

M305-1ML, Plant Protease Inhibitor Cocktail: Preparation of 100X Solution: 1. Add 1 ml deionized, distilled water and mix gently until powder is completely ...

mix. Aliquot into multiple tubes and store at -20°C. *Solution may be cloudy and precipitate may be present. 1 mL inhibitor is sufficient to inhibit 20 mL ...

Protease Inhibitor Cocktail: 5 mM EDTA (0.5 M, pH 8 stock, RT). 2 mM EGTA (0.2 M, pH 8 stock, RT). 0.2 mM 4-(2-aminoethyl)benzene sulfonyl fluoride ...

Protease Inhibitor Cocktail (1000×) ; Antipain, 5 mg ; DMSO, 7 mL ; H2O · 3 mL ; Combine in a 15-mL screw-cap polypropylene tube and mix overnight at room ...

2015年10月15日 — Mix by pipetting up and down and continue with cell lysis. Tips. • This protease inhibitor cocktail can also be incorporated into a lysis buffer ...

Protease inhibitors and protease inhibitor cocktails are a useful reagent during protein research applications. In this article, we'll take a look at what ...

Solutions and Reagents: The Protease Inhibitor Cocktail (100X) is composed of a proprietary mix of AEBSF, Aprotinin, Bestatin,. E64, Leupeptin, and Pepstatin ...

A cocktail of five protease inhibitors with broad specificity for the inhibition of aspartic, cysteine, serine, and metalloproteases as well as aminopeptidases.