IPTG induction using the lac promoter | 私立大學五星教授網

IPTG induction is a method of regulating protein synthesis by triggering transcription of the lac operon.

IPTG是一種分子生物學試劑，全稱異丙基-β-D-硫代半乳糖苷（英語：Isopropyl β-D-1-thiogalactopyranoside），是異乳糖的結構類似物，能與乳糖抑制子結合，高效啟動乳糖操縱 ...

IPTG (isopropylthio-β-galactoside) is an inducer of β-galactosidase activity in bacteria and is suitable for use with X-gal or bluo-gal to detect lac gene ...

由 L Gomes 著作 · 2020 · 被引用 68 次 — IPTG induction had a negative effect not only on growth but also on cellular viability of E. coli suspended cultures. Although IPTG was added in ...

IPTG is a structural mimic of lactose (it resembles the galactose sugar) that also binds to the lac repressor and induces a similar conformational change.

由 RG Simas 著作 · 2023 · 被引用 4 次 — Coupling transcription of a cloned gene to the lac operon with induction by isopropylthio-β-galactoside (IPTG) has been a favoured approach for recombinant ...

IPTG induction is a longstanding technique in molecular biology. In this article, you'll take a deep dive into this important application.

2023年7月19日 — The basic principle behind recombinant protein expression is that we can stick the genetic instructions for a protein we want made into ...

由 A Fernández-Castané 著作 · 2012 · 被引用 36 次 — By increasing the extracellular inducer, IPTG can enter the cell by diffusion and/or active transport and can accumulate until it reaches a ...

IPTG is an effective inducer of protein expression in the concentration range of 100 μM to 1.0 mM. Concentration used depends on the strength of induction ...