This tutorial describes the use of the NEBioCalculator web tool to optimize the molar ratio between vector and insert DNA for use in a ligation reaction.
This reaction, called ligation, is performed by the T4 DNA ligase enzyme. The DNA ligase catalyzes the formation of covalent phosphodiester linkages, which ...
DNA ligases are essential enzymes with critical roles in DNA replication and repair. In the lab, DNA ligation is performed for both cloning and non-cloning ...
Summary of ligation tips · Consider your cloning strategy · Check the ends of your DNA inserts · Set up ideal reaction conditions · Avoid inhibitors · Visualize ...
DNA Ligation is the joining of 2 DNA molecules by the enzyme, DNA ligase. DNA ligase catalyzes the formation of two covalent phosphodiester bonds between the 3' ...
Ligation is the joining of two nucleotides, or two nucleic acid fragments, into a single polymeric chain through the action of an enzyme known as a ligase.
Thermo Scientific Rapid DNA Ligation Kit enables fast sticky-end or blunt-end DNA ligation in only 5 minutes at room temperature.The kit contains T4 DNA ...
This tutorial describes the use of the NEBioCalculator web tool to optimize the molar ratio between vector and insert DNA for use in a ligation reaction.
