由 I Manual 著作 — The BL21(DE3)pLysS competent cells provide tighter control of protein expression for expression of toxic proteins and are resistant to chloramphenicol. When ...
由 I Manual 著作 — The BL21(DE3)pLysS competent cells provide tighter control of protein expression for expression of toxic proteins and are resistant to chloramphenicol. When ...
Note: If you are using BL21(DE3)pLysS or. BL21(DE3)pLysE, you may choose not to include chloramphenicol in these cultures. Generally, the cells will not lose ...
The pLysS plasmid contains a chloramphenicol resistant gene and a p15A replication origin which is compatible with those found in pBR322 and pUC derived ...
2017年2月22日 — BL21(DE3) pLysS strains have a pLysS plasmid which is used to reduce basal protein expression, and chloramphenicol is required to maintain the ...
The T7 lysozyme is expressed from a plasmid conferring resistance to chloramphenicol. 69451 will need to be cultured with chloramphenicol to maintain this ...
Note: If you are using BL21 Star™(DE3)pLysS, you may choose not to include chloramphenicol in these cultures. Generally, the cells will not lose the pLysS.