Lentivirus transduction | 私立大學五星教授網

慢病毒載體是藉由對哺乳動物細胞的多質體轉染(multi-plasmid transfection)在體外產生的逆轉錄(又稱反轉錄)病毒。逆轉錄病毒顆粒從培養. 基中收穫，用於將轉基因穩定插入到 ...

Detailed procedure for how to perform a lentiviral transduction of MISSION shRNA lentiviral particles to achieve a stable long term silencing and phenotypic ...

Lentiviral particles can efficiently infect a broad range of cell types, including both dividing and nondividing cells.

由 J Elegheert 著作 · 2018 · 被引用 193 次 — Lentiviral transduction is an efficient method for the delivery of transgenes to mammalian cells and unifies the ease of use and speed of transient transfection ...

由 J Elegheert 著作 · 2018 · 被引用 193 次 — Lentiviral transduction is an efficient method for the delivery of transgenes to mammalian cells and unifies the ease of use and speed of ...

This protocol can be used to produce lentivirus from a lentiviral vector transfected into 293T cells using a polyethylenimine (PEI) transfection protocol.

Lentiviral vectors have been successfully used to transduce most cell types within the central nervous system (CNS) in vivo, including neurons, astrocytes, ...