Lentivirus transfection protocol | 私立大學五星教授網

This protocol can be used to produce lentivirus from a lentiviral vector transfected into 293T cells using a polyethylenimine (PEI) transfection protocol.

Transfection protocol · In 1.5 mL tubes, mix 600 μL OptiMEM with 30μL FuGENE. · In the cap of the tube, mix 4μg master mix with 4μg lentivirus construct. · Close ...

If viral toxicity is observed in your cell line, you may decrease the infection time to between 4 to 20 hours. Remove the virus-containing media and replace ...

Aliquot and Store the concentrated lentivirus at -80oC. Avoid multi-cycle of freeze and thaw. One cycle will lead to 50-90% loss of lentivirus.

Protocol for 12-well plate format. Lentiviral production consists of the following steps: Day 1 Seed 293T packaging cells.

Detailed procedure for how to perform a lentiviral transduction of MISSION shRNA lentiviral particles to achieve a stable long term silencing and phenotypic ...

由 LY Brown 著作 · 2020 · 被引用 30 次 — We present a calcium phosphate-based protocol for lentiviral production and concentration for in vitro and in vivo use.

1. 24 hours before transfection, subculture your 293T cells for packaging in such a way that they will be 70 - 75% confluence at the time of transfection.

2024年2月1日 — This study presents a gene transfer protocol using a lentiviral vector for human ILC2s. This allowed us to increase or decrease gene expression.